FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Doa, Cpsf6
FlyBase ID
FBig0000116689
Interaction Network
Interactions Browser links
Doa network
Cpsf6 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0238799-4.coIP.WB
Reference
Tang et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Cpsf6 

CPSF6

bait

GFP tag

Cpsf6 
Doa 

DOA

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cpsf6 
RS domain
necessary binding region
aa 401-652

coordinates relative to CG7185-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tang et al., 2018)
FBrf0238799-6.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Doa 

DOA

enzyme

HA tag

Doa 

DOA

enzyme

HA tag

Cpsf6 

CPSF6

enzyme target

GST tag, P[32] label

Cpsf6 

CPSF6

enzyme target

GST tag, P[32] label

Cpsf6 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)