FB2020_06, released Dec 22, 2020

Physical Interaction report

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General Information
Interaction Type
physical association
Interacting Genes
mir-210, mnd
FlyBase ID
FBig0000116711
Interaction Network
Interactions Browser links
mir-210 network
mnd network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0239602-1.miR-mRNA.LUC
Reference
Cusumano et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
mir-210 

miR-210

bait
mnd 

mnd

prey
mnd 
mnd 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mnd 
3-prime UTR
sufficient binding region
mnd 
mir-210 target site
mutation decreasing interaction
ACGCACAAC

deletion in the mir-210 target site, ACGCACAAC > ACGC----C

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Luciferase reporter assay.

(Cusumano et al., 2018)

Source was S2R+ cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

(Cusumano et al., 2018)
FBrf0239602-3.miR-mRNA.PCR
Reference
Cusumano et al., 2018
Description
physical association
Assay
Collection
Source/Stage
brain / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-210 

mir-210

bait
mnd 

mnd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult brains of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.

(Cusumano et al., 2018)

Overexpression of mir-210 reduces mnd mRNA levels.

(Cusumano et al., 2018)
External Crossreferences and Linkouts ( 0 )
References (1)