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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000116741
Interaction Network
Interactions Browser links
ix network
dsx network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0204143-2.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dsx 

DSX

bait

fused to GAL4 DNA-binding domain

dsx 
dsx 
ix 

IX

prey

fused to GAL4 activation domain

ix 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dsx 
interaction domain
sufficient binding region
aa 1-412

coordinates relative to dsx-PC

ix 
interaction domain
mutation disrupting interaction
aa 102aa 109aa 110aa 116

H102A

C109A

C110A

H116A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

FBrf0204143-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ix 

IX

bait

HA tag

dsx 

DSX

prey

V5 tag

dsx 
dsx 
dsx 
dsx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dsx 
interaction domain
sufficient binding region
aa 1-406

coordinates relative to dsx-PC

dsx 
interaction domain
necessary binding region
aa 398-406

coordinates relative to dsx-PC

dsx 
interaction domain
mutation disrupting interaction
aa 400aa 403

Y400A, coordinates relative to dsx-PC

N403A, coordinates relative to dsx-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)