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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000117677
Interaction Network
Interactions Browser links
Ilp2 network
ImpL2 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0204716-1.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ilp2 

Dilp

bait

FLAG tag

Imp-L2

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
interaction domain
mutation disrupting interaction
aa 232

stop codon introduced at aa 232, coordinates relative to ImpL2-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0212768-1.FW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

ImpL2

bait

Myc tag

Ilp2 

Ilp2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait secreted into S2 cell culture medium; prey derived from wild-type adult head extract.

Adult head proteins were separated by PAGE, transferred to nitrocellulose and probed with Myc-tagged ImpL2. Ilp2 identified by disappearance of band in Ilp2 mutant extracts.

FBrf0218395-3209.DPiM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
unspecified role
unspecified role
unspecified role
Ilp2 
unspecified role
Ilp2 
unspecified role
Ilp2 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 317.04122

FBrf0220509-8.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ilp2 

Dilp2

bait

His tag

Imp-L2

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

ImpL2 binds most Ilp proteins tested, except for Ilp7.

The Sdr and ImpL2 bind Ilp family proteins with different binding affinities.

Sdr and ImpL2 do not bind each other.

ImpL2 and Sdr compete for binding to Ilp2.

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0240146-5.SPR
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Imp-L2

bait
Ilp2 

DILP2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and Sf9 system; prey produced as a yeast expression system.

External Crossreferences and Linkouts ( 0 )
References (5)