FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
AGO1, TER94
FlyBase ID
FBig0000120034
Interaction Network
Interactions Browser links
AGO1 network
TER94 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0243075-1.coIP.WB
Reference
Kobayashi et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
AGO1 

Ago1

bait

FLAG tag

AGO1 
TER94 

VCP

prey

SBP tag

TER94 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
TER94 
N-terminal domain
necessary binding region
unspecified
AGO1 
ubiquitination target residue 514
mutation decreasing interaction
aa 514

K514R, coordinates relative to AGO1-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

A constitutively empty mutant of AGO1, Y619E, was used for interaction assays.

(Kobayashi et al., 2019)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kobayashi et al., 2019)
FBrf0253135-1.coIP.WB
Reference
Gao et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait
TER94 

VCP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gao et al., 2022)
FBrf0253135-2.coIP.WB
Reference
Gao et al., 2022
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait
TER94 

VCP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gao et al., 2022)
FBrf0253135-3.coIP.WB
Reference
Gao et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait

Myc tag

TER94 

VCP

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gao et al., 2022)
FBrf0253135-4.coIP.WB
Reference
Gao et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
TER94 

VCP

bait

FLAG tag

AGO1 

AGO1

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gao et al., 2022)
FBrf0253135-5.coIP.WB
Reference
Gao et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait

Flag tag

AGO1 
TER94 

VCP

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AGO1 
C terminal interaction domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gao et al., 2022)
FBrf0253135-6.coIP.WB
Reference
Gao et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
TER94 

VCP

bait

Flag tag

TER94 
AGO1 

AGO1

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
TER94 
ATPase domains
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gao et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (2)