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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000120514
Interaction Network
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Mad network
Cdk8 network
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0245883-1.PD.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdk8 

CDK8

bait

GST tag

Mad 

Mad

prey

His tag

Mad 
Mad 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mad 
MH1 and linker domains
sufficient binding region
aa 1-230

coordinates relative to Mad-PA

Mad 
linker and MH2 domains
sufficient binding region
aa 151-455

coordinates relative to Mad-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0245883-2.Y2H
Reference
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mad 

Mad

bait

fused to GAL4 DNA-binding domain

Mad 
Cdk8 

CDK8

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mad 
MH1 and linker domains
sufficient binding region
aa 1-230

coordinates relative to Mad-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

External Crossreferences and Linkouts ( 0 )
References (1)