FB2021_01, released Feb 18, 2021

Physical Interaction report

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General Information
Interaction Type
physical association
Interacting Genes
Klp61F, galla-2
FlyBase ID
FBig0000120575
Interaction Network
Interactions Browser links
Klp61F network
galla-2 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0246133-3.coIP.WB
Reference
Hwang et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Klp61F 

Klp61F

bait

FLAG tag

galla-2 

Galla-2

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hwang et al., 2020)
FBrf0246133-5.PD.WB
Reference
Hwang et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
galla-2 

Galla-2

bait

GST tag

Klp61F 

Klp61F

prey

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Hwang et al., 2020)
FBrf0246133-6.coIP.WB
Reference
Hwang et al., 2020
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp61F 

Klp61F

bait
galla-2 

Galla-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Hwang et al., 2020)
FBrf0246133-7.coIP.WB
Reference
Hwang et al., 2020
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp61F 

Klp61F

bait
galla-2 

Galla-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was syncytial embryos of transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Hwang et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (1)