FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
hbs, rst
FlyBase ID
FBig0000120695
Interaction Network
Interactions Browser links
hbs network
rst network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0187402-1.coIP.WB
Reference
Bao and Cagan, 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
hbs 

Hibris

bait

V5 tag

rst 

Roughest

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bao and Cagan, 2005)
FBrf0222867-175.ELISA
Reference
Ozkan et al., 2013
Description
physical association
Assay
Collection
ECIA
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
rst 

rst

bait

Fc tag

rst 
hbs 

hbs

prey

alkaline phosphatase tag, COMP pentamerization motif

hbs 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
rst 
extracellular domain
sufficient binding region
hbs 
extracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.

(Ozkan et al., 2013)
FBrf0222867-89.ELISA
Reference
Ozkan et al., 2013
Description
physical association
Assay
Collection
ECIA
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
hbs 

hbs

bait

Fc tag

hbs 
rst 

rst

prey

alkaline phosphatase tag, COMP pentamerization motif

rst 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hbs 
extracellular domain
sufficient binding region
rst 
extracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.

(Ozkan et al., 2013)
External Crossreferences and Linkouts ( 0 )
References (2)