FB2025_05 , released December 11, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mxc, FLASH
FlyBase ID
FBig0000121028
Interaction Network
Interactions Browser links
mxc network
FLASH network
Reported Interactions
FBrf0248673-1.coIP.WB
Reference
Kemp et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mxc 

Mxc

bait
FLASH 

FLASH

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of Kc cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kemp et al., 2021)
FBrf0248673-3.PD.A
Reference
Kemp et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
FLASH 

FLASH

bait

GST tag

FLASH 
mxc 

Mxc

prey

[35]S label

mxc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
FLASH 
C-terminal domain
sufficient binding region
aa 667-844

coordinates relative to FLASH-PA, described as last 178aa of FLASH

mxc 
C-terminal domain
sufficient binding region
aa 1542-1745

coordinates relative to mxc-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Kemp et al., 2021)
External Crossreferences and Linkouts ( 0 )
References (1)