FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
AGO1, Ltn1
FlyBase ID
FBig0000121739
Interaction Network
Interactions Browser links
AGO1 network
Ltn1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0253135-12.coIP.WB
Reference
Gao et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait
Ltn1 

Ltn1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gao et al., 2022)
FBrf0253135-13.coIP.WB
Reference
Gao et al., 2022
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait
Ltn1 

Ltn1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gao et al., 2022)
FBrf0253135-14.coIP.WB
Reference
Gao et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait

FLAG tag

AGO1 
AGO1 
Ltn1 

Ltn1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AGO1 
LBM1 domain
mutation decreasing interaction
aa 175-182

delta LBM1 aa 175-182

AGO1 
N and Paz domains
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from tagged transfected construct; prey produced from endogenous gene.

(Gao et al., 2022)

Ltn1 preferentially interacts with ER targeted AGO1.

(Gao et al., 2022)
FBrf0253135-15.coIP.WB
Reference
Gao et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Ltn1 

Ltn1

bait

FLAG tag

AGO1 

AGO1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gao et al., 2022)

All Ltn1 domains interact with AGO1 however, Ltn1 heat repeat domain residues 11-17 interact most robustly.

(Gao et al., 2022)
FBrf0253135-16.CL.FM
Reference
Gao et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait

fused to outer mitochondrial membrane anchor

Ltn1 

Ltn1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction detected by the ddFP system; RA is a quenched chromophore in a monomeric state, GB is able to increase fluorescence of RA when a heterodimer is formed.

(Gao et al., 2022)

Source was live, human U2OS cells; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Gao et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (1)