FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
cac, brp
FlyBase ID
FBig0000121977
Interaction Network
Interactions Browser links
cac network
brp network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0255785-1.Y2H
Reference
Ghelani et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cac 

Cac

bait

fused to GAL4 DNA-binding domain

cac 
cac 
brp 

BRP

prey

fused to GAL4 activation domain

brp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cac 
C terminal domain
mutation decreasing interaction
unspecified

Deletion of the last 130 aa.

cac 
C terminal domain
mutation disrupting interaction
unspecified

Deletion of the last 304 aa.

brp 
N terminal domain
sufficient binding region
aa 1-320
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Ghelani et al., 2023)

Two-hybrid system: yeast GAL4-BD/GAL4-AD. cac isoform used not specified, thus unable to give exact aa locations of deletions; the region between 304 aa and 130 aa from the end are necessary for interaction.

(Ghelani et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (1)