Open Close
General Information
Name
AT_cDNA
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000001
Project
Created by
[BDGP: cDNA & EST FAQ](http://www.fruitfly.org/EST/faq.html)
Vector
Title
AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
Accessions
Overview
Description
Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.
Available from
Biosample Source
Overview
Other tissues studied
Cell component
Cell line
Key genes
Methods
Sample preparation
Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.
Biosamples used (1)
Biosample
Type
Title
D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle, source for cDNA.
Reagent Details
Molecular Construct used
Protocol
RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.
Mode of Assay
Data analysis
Comments
Associated Data
Size
Associated features
22,564 Clone(s)
Files
Additional Information
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Name Synonyms
AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
Secondary FlyBase IDs
    References (1)