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General Information
Name
CK_cDNA
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000003
Project
Created by
[BDGP: cDNA & EST FAQ](http://www.fruitfly.org/EST/faq.html)
Title
CK cDNA library of D. melanogaster, Oregon-R strain, embryo (8-16hr), endoplasmic reticulum.
Accessions
Overview
Description

Cloned cDNAs prepared from polyadenylated RNA isolated from embryonic rough ER, to enrich for mRNAs encoding secreted and transmembrane proteins; also enriched for rare cDNAs.

Available from
Biosample Source
Overview
Sex
Other tissues studied
Cell line
Key genes
Methods
Sample preparation

Rough endoplasmic reticulum (ER) was isolated from 8-16 hr AEL embryos from a non-isogenic Oregon R population cage to enrich for mRNA encoding secreted and membrane-associated proteins.

Biosamples used (1)
Biosample
Type
Title
D. melanogaster, Oregon-R strain, embryo (8-16hr), endoplasmic reticulum, source for cDNA.
Reagent Details
Molecular Construct used
Protocol

RNA from isolated endoplasmic reticulum as poly(A)+ selected twice. Note that only 70% of the cDNAs appear to be from secreted or membrane-associated proteins; the rest are cytoplasmic or nuclear proteins. Oligo(dT) primed with PstI site at end of primer for first strand synthesis; normalized to genomic DNA beads; KS complementary sequence put at 5' end of each cDNA HindIII/XmnI adapter on 5' ends of clones; cDNA directionally cloned into HindIII/PstI-digested pBluescript SK(+) vector.

Mode of Assay
Data analysis
Comments
Associated Data
Size
Associated features
1,033 Clone(s)
Files
Additional Information
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Name Synonyms
CK cDNA library of D. melanogaster, Oregon-R strain, embryo (8-16hr), endoplasmic reticulum.
Secondary FlyBase IDs
    References (1)