FB2020_06, released Dec 22, 2020

Reagent: EC_cDNA

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General Information
Name
EC_cDNA
Species
D. melanogaster
Reagent type
cDNA library
FlyBase ID
FBlc0000004
Project
Created by
Marie Lagueux, Hoffmann lab
Vector
Title
Exelexis EC cDNA library of bacterially challenged D. melanogaster, iso-1 strain, larva, fat body.
Accessions
Overview
Description

Cloned cDNAs prepared from polyadenylated RNA isolated from fat bodies of immunologically challenged third instar larvae. The strain used was not the iso-1 sequenced strain.

(Carlson, 2005.9.12)
Available from
Exelixis cDNA clones are not available for distribution.
(Carlson, 2005.9.12)
Biosample Source
Overview
Strain
Stage
larval stage
(Carlson, 2005.9.12)
Sex
Tissue isolated
embryonic/larval fat body
(Carlson, 2005.9.12)
Other tissues studied
Cell component
Cell line
Key genes
Methods
biotic treatment
(Carlson, 2005.9.12)
Sample preparation

The fat body was dissected from third instar larva challenged with gram positive and gram negative bacteria.

Biosamples used (1)
Biosample
Type
Title
BS_EC_cDNA
tissue
D. melanogaster, third instar larva, fat body, source for cDNA.
Reagent Details
Methods
poly(A) RNA isolation
(Carlson, 2005.9.12)
oligo-dT priming
(Carlson, 2005.9.12)
Molecular Construct used
Protocol

RNA was isolated from fat body from 3rd instar larva challenged with gram+/- bacteria; cDNA was oligo(dT) primed.

(Carlson, 2005.9.12)
Mode of Assay
Data analysis
Comments

The Exelixis ESTs were submitted to GenBank by the BDGP on behalf of Exelixis.

(Carlson, 2005.9.12)
Associated Data
Size
Associated features
12,692 Clone(s)
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Files
Additional Information

[BDGP: other ESTs](http://www.fruitfly.org/EST/otherEST.html)

Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
EC
EC_cDNA
ML01
(Chen et al., 2005)
Name Synonyms
Exelexis EC cDNA library of bacterially challenged D. melanogaster, iso-1 strain, larva, fat body.
(Carlson, 2005.9.12)
Secondary FlyBase IDs
    References (2)