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FB2019_05, released Oct 15, 2019
Reagent: EC_cDNA
FB2019_05, released Oct 15, 2019
Reagent: EC_cDNA
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General Information
Name
EC_cDNA
Species
D. melanogaster
Reagent type
cDNA library
FlyBase ID
FBlc0000004
Project
BDGP_cDNA
Created by
Marie Lagueux, Hoffmann lab
Vector
pSport1-Tag21
Title
Exelexis EC cDNA library of bacterially challenged D. melanogaster, iso-1 strain, larva, fat body.
Accessions
15896
Overview
Description
Cloned cDNAs prepared from polyadenylated RNA isolated from fat bodies of immunologically challenged third instar larvae. The strain used was not the
iso-1
sequenced strain.
(
Carlson, 2005.9.12
)
Available from
Exelixis cDNA clones are not available for distribution.
(
Carlson, 2005.9.12
)
Biosample Source
Overview
Strain
Stage
larval stage
(
Carlson, 2005.9.12
)
Sex
Tissue isolated
embryonic/larval fat body
(
Carlson, 2005.9.12
)
Other tissues studied
Cell component
Cell line
Key genes
Methods
biotic treatment
(
Carlson, 2005.9.12
)
Sample preparation
The fat body was dissected from third instar larva challenged with gram positive and gram negative bacteria.
Biosamples used (1)
Export to HitList
Biosample
Type
Title
BS_EC_cDNA
tissue
D. melanogaster, third instar larva, fat body, source for cDNA.
Reagent Details
Methods
poly(A) RNA isolation
(
Carlson, 2005.9.12
)
oligo-dT priming
(
Carlson, 2005.9.12
)
Molecular Construct used
pSport1-Tag21
Protocol
RNA was isolated from fat body from 3rd instar larva challenged with gram+/- bacteria; cDNA was oligo(dT) primed.
(
Carlson, 2005.9.12
)
Mode of Assay
Data analysis
Comments
The Exelixis ESTs were submitted to GenBank by the BDGP on behalf of Exelixis.
(
Carlson, 2005.9.12
)
Associated Data
Size
Associated features
12,692 Clone(s)
Download
Export to HitList
Files
Additional Information
[BDGP: other ESTs](http://www.fruitfly.org/EST/otherEST.html)
Related Datasets
Parent projects (1)
Export to HitList
Project
Type
Title
BDGP_cDNA
reagent
BDGP generated cDNA and EST libraries.
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
EC
EC_cDNA
ML01
(
Chen et al., 2005
)
Name Synonyms
Exelexis EC cDNA library of bacterially challenged D. melanogaster, iso-1 strain, larva, fat body.
(
Carlson, 2005.9.12
)
Secondary FlyBase IDs
References (2)
Report Sections
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Close
General Information
Overview
Biosample Source
Reagent Details
Associated Data
Related Datasets
Synonyms and Secondary IDs
References