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General Information
Name
GH_cDNA
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000007
Project
Created by
[BDGP: cDNA & EST FAQ](http://www.fruitfly.org/EST/faq.html)
Vector
Title
GH cDNA library of D. melanogaster, iso-1 strain, adult head.
Accessions
Overview
Description

Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

Available from
Biosample Source
Overview
Sex
Tissue isolated
Other tissues studied
Cell component
Cell line
Key genes
Methods
Sample preparation

Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

Biosamples used (1)
Biosample
Type
Title
D. melanogaster, iso-1 strain, adult head, source for cDNA.
Reagent Details
Molecular Construct used
Protocol

RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

Mode of Assay
Data analysis
Comments

The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

Associated Data
Size
Associated features
20,768 Clone(s)
Files
Additional Information
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Name Synonyms
GH cDNA library of D. melanogaster, iso-1 strain, adult head.
Secondary FlyBase IDs
    References (1)