FB2025_01 , released February 20, 2025
Reagent: GH_cDNA
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General Information
Name
GH_cDNA
Species
D. melanogaster
Reagent type
cDNA library
FlyBase ID
FBlc0000007
Project
Created by
[BDGP: cDNA & EST FAQ](http://www.fruitfly.org/EST/faq.html)
Vector
Title
GH cDNA library of D. melanogaster, iso-1 strain, adult head.
Accessions
Overview
Description

Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

(Rubin et al., 2000)
Available from
Biosample Source
Overview
Strain
iso-1
(Rubin et al., 2000)
Stage
adult stage
(Rubin et al., 2000)
Sex
Tissue isolated
adult head
(Rubin et al., 2000)
Other tissues studied
Cell component
Cell line
Key genes
Methods
Sample preparation

Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

Biosamples used (1)
Biosample
Type
Title
BS_GH_cDNA
tissue
D. melanogaster, iso-1 strain, adult head, source for cDNA.
Reagent Details
Methods
poly(A) RNA isolation
(Rubin et al., 2000)
oligo-dT priming
(Rubin et al., 2000)
size fractionation
(Rubin et al., 2000)
Molecular Construct used
Protocol

RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

(Rubin et al., 2000)
Mode of Assay
Data analysis
Comments

The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

(Rubin et al., 2000)
Associated Data
Size
Associated features
20,768 Clone(s)
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Files
Additional Information
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
GH
(Rubin et al., 2000)
GH_cDNA
Name Synonyms
GH cDNA library of D. melanogaster, iso-1 strain, adult head.
(Rubin et al., 2000)
Secondary FlyBase IDs
    References (1)