Dataset GM_pBS_cDNA cDNA library

General Information
Name	GM_pBS_cDNA	Species	D. melanogaster
Dataset type	cDNA library	FlyBase ID	FBlc0000008
Source & Content
Consists of	cDNA clones in plasmid vector (Rubin et al., 2000)
Created by	BDGP: cDNA & EST FAQ
Available from	Selected clones available from the DGRC; other sources listed at BDGP, Materials. BDGP: Materials DGRC: Vectors, cDNAs, ESTs
Strain	Oregon-R (Rubin et al., 2000)
Stage & tissue
Stage Tissue/Position (including subcellular localization) Reference oogenesis stage S1 -- S6 & adult stage \| female ovary (Rubin et al., 2000)
Cell Line
Recent Updates
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FB2013_03
FB2013_02
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Description & Members
Description	Cloned cDNAs prepared from polyadenylated RNA isolated from stage S1-S6 ovaries. (Rubin et al., 2000)
Parent collections
Component collection(s)
Number in collection
Comment on number in collection
Members
Experimental protocol
Vector	pBluescript_SK(-) (Rubin et al., 2000)
Sample preparation	mRNA source--ovaries, stage 1-6 of oogenesis, from a non-isogenic Oregon R strain P2 population cage, polyA+ selected once, RNA provided by Allan Spradling. (Rubin et al., 2000)
Collection preparation	cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones GM01001-GM11496, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid. (Rubin et al., 2000)
Mode of assay
Assay platform
Data analysis
Additional data
	More information is available under: UniGene: D. melanogaster GM_pBS
Associated files
Additional sites
Synonyms & Secondary IDs
Reported As
Symbol Synonym	GM (Rubin et al., 2000) GM_pBS (Rubin et al., 2000, ) GM_pBS_cDNA
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]