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General Information
Name
GM_pBS_cDNA
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000008
Project
Created by
[BDGP: cDNA & EST FAQ](http://www.fruitfly.org/EST/faq.html)
Title
GM cDNA library of D. melanogaster, Oregon-R strain, adult female, ovary, pBS vector.
Accessions
Overview
Description
Cloned cDNAs prepared from polyadenylated RNA isolated from stage S1-S6 ovaries.
Available from
Biosample Source
Overview
Tissue isolated
Other tissues studied
Cell component
Cell line
Key genes
Methods
Sample preparation
Ovaries at stage 1-6 of oogenesis were collected from a non-isogenic Oregon R strain P2 population cage.
Biosamples used (1)
Biosample
Type
Title
D. melanogaster, Oregon-R strain, adult female, ovary, source for cDNA.
Reagent Details
Molecular Construct used
Protocol
RNA from ovaries was poly(A)+ selected once (provided by A. Spradling). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones GM01001-GM11496, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
Mode of Assay
Data analysis
Comments
Associated Data
Size
Associated features
5,044 Clone(s)
Files
Additional Information
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Name Synonyms
GM cDNA library of D. melanogaster, Oregon-R strain, adult female, ovary, pBS vector.
Secondary FlyBase IDs
    References (1)