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General Information
Name
GM_pOT2_cDNA
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000009
Project
Created by
[BDGP: cDNA & EST FAQ](http://www.fruitfly.org/EST/faq.html)
Vector
Title
GM cDNA library of D. melanogaster, Oregon-R strain, adult female, ovary, pOT2 vector.
Accessions
Overview
Description

Cloned cDNAs prepared from polyadenylated RNA isolated from stage S1-S6 ovaries.

Available from
Biosample Source
Overview
Tissue isolated
Other tissues studied
Cell component
Cell line
Key genes
Methods
Sample preparation

Ovaries at stage 1-6 of oogenesis were collected from a non-isogenic Oregon R strain P2 population cage.

Biosamples used (1)
Biosample
Type
Title
D. melanogaster, Oregon-R strain, adult female, ovary, source for cDNA.
Reagent Details
Molecular Construct used
Protocol

RNA from ovaries was poly(A)+ selected once (provided by A. Spradling). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones GM12101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.

Mode of Assay
Data analysis
Comments
Associated Data
Size
Associated features
5,478 Clone(s)
Files
Additional Information
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
GM_pOT2_cDNA
Name Synonyms
GM cDNA library of D. melanogaster, Oregon-R strain, adult female, ovary, pOT2 vector.
Secondary FlyBase IDs
    References (1)