Dataset HL_pBS_cDNA cDNA library

General Information
Name	HL_pBS_cDNA	Species	D. melanogaster
Dataset type	cDNA library	FlyBase ID	FBlc0000010
Source & Content
Consists of	cDNA clones in plasmid vector (Rubin et al., 2000)
Created by	BDGP: cDNA & EST FAQ
Available from	Selected clones available from the DGRC; other sources listed at BDGP, Materials. BDGP: Materials DGRC: Vectors, cDNAs, ESTs
Strain	iso-1 (Rubin et al., 2000)
Stage & tissue
Stage Tissue/Position (including subcellular localization) Reference adult stage adult head (Rubin et al., 2000)
Cell Line
Recent Updates
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FB2013_03
FB2013_02
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Description & Members
Description	Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads. (Rubin et al., 2000)
Parent collections
Component collection(s)
Number in collection
Comment on number in collection
Members
Experimental protocol
Vector	pBluescript_SK(-) (Rubin et al., 2000)
Sample preparation	mRNA source--adult heads, from an isogenic y; cn bw sp strain, polyA+ selected once. (Rubin et al., 2000)
Collection preparation	cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones HL01001-HL07796, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid. (Rubin et al., 2000)
Mode of assay
Assay platform
Data analysis
Additional data
	More information is available under: UniGene: D. melanogaster HL_pBS
Associated files
Additional sites
Comments
	The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made. (Rubin et al., 2000)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	HL_pBS (Rubin et al., 2000, ) HL_pBS_cDNA
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]