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General Information
Name
LD_pBS_cDNA
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000012
Project
Created by
[BDGP: cDNA & EST FAQ](http://www.fruitfly.org/EST/faq.html)
Title
LD cDNA library of D. melanogaster, iso-1 strain, embryo (0-22 hr), pBS vector.
Accessions
Overview
Description
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Available from
Biosample Source
Overview
Stage
Sex
Tissue isolated
Other tissues studied
Cell component
Cell line
Key genes
Methods
Sample preparation
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
Biosamples used (1)
Biosample
Type
Title
D. melanogaster, iso-1 strain, embryo (0-22 hr), source for cDNA.
Reagent Details
Molecular Construct used
Protocol
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
Mode of Assay
Data analysis
Comments
Associated Data
Size
Associated features
13,843 Clone(s)
Files
Additional Information
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Name Synonyms
LD cDNA library of D. melanogaster, iso-1 strain, embryo (0-22 hr), pBS vector.
Secondary FlyBase IDs
    References (1)