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General Information
Name
LD_pOT2_cDNA
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000013
Project
Created by
[BDGP: cDNA & EST FAQ](http://www.fruitfly.org/EST/faq.html)
Vector
Title
LD cDNA library of D. melanogaster, iso-1 strain, embryo (0-22 hr), pOT2 vector.
Accessions
Overview
Description
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Available from
Biosample Source
Overview
Stage
Sex
Tissue isolated
Other tissues studied
Cell component
Cell line
Key genes
Methods
Sample preparation
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
Biosamples used (1)
Biosample
Type
Title
D. melanogaster, iso-1 strain, embryo (0-22 hr), source for cDNA.
Reagent Details
Molecular Construct used
Protocol
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
Mode of Assay
Data analysis
Comments
Associated Data
Size
Associated features
18,878 Clone(s)
Files
Additional Information
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
LD_pOT2_cDNA
Name Synonyms
LD cDNA library of D. melanogaster, iso-1 strain, embryo (0-22 hr), pOT2 vector.
Secondary FlyBase IDs
    References (1)