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General Information
Name
LP_cDNA
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000014
Project
Created by
[BDGP: cDNA & EST FAQ](http://www.fruitfly.org/EST/faq.html)
Vector
Title
LP cDNA library of D. melanogaster, iso-1 strain, larva and pupa.
Accessions
Overview
Description
Cloned cDNAs prepared from polyadenylated RNA isolated from larvae and early pupae.
Available from
Biosample Source
Overview
Sex
Tissue isolated
Other tissues studied
Cell component
Cell line
Key genes
Methods
Sample preparation
Larvae and early pupae at various stages of development were collected from an isogenic y; cn bw sp (iso-1) strain.
Biosamples used (1)
Biosample
Type
Title
D. melanogaster, iso-1 strain, larva and pupa, source for cDNA.
Reagent Details
Molecular Construct used
Protocol
RNA was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~500,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).
Mode of Assay
Data analysis
Comments
Associated Data
Size
Associated features
15,074 Clone(s)
Files
Additional Information
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Name Synonyms
LP cDNA library of D. melanogaster, iso-1 strain, larva and pupa.
Secondary FlyBase IDs
    References (1)