Dataset LP_cDNA cDNA library

General Information
Name	LP_cDNA	Species	D. melanogaster
Dataset type	cDNA library	FlyBase ID	FBlc0000014
Source & Content
Consists of	cDNA clones in plasmid vector (Rubin et al., 2000)
Created by	BDGP: cDNA & EST FAQ
Available from	Selected clones available from the DGRC; other sources listed at BDGP, Materials. BDGP: Materials DGRC: Vectors, cDNAs, ESTs
Strain	iso-1 (Rubin et al., 2000)
Stage & tissue
Stage Tissue/Position (including subcellular localization) Reference larval stage organism (Rubin et al., 2000) pupal stage organism (Rubin et al., 2000) Comment:"early pupae"
Cell Line
Recent Updates
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FB2013_03
FB2013_02
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Description & Members
Description	Cloned cDNAs prepared from polyadenylated RNA isolated from larvae and early pupae. (Rubin et al., 2000)
Parent collections
Component collection(s)
Number in collection
Comment on number in collection
Members
Experimental protocol
Vector	pOT2 (Rubin et al., 2000)
Sample preparation	mRNA source--varying stages of larvae and early pupae, from an isogenic y; cn bw sp strain, polyA+ selected once. (Rubin et al., 2000)
Collection preparation	Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~500,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL). (Rubin et al., 2000)
Mode of assay
Assay platform
Data analysis
Additional data
	More information is available under: UniGene: D. melanogaster LP_pOT2
Associated files
Additional sites
Synonyms & Secondary IDs
Reported As
Symbol Synonym	LP (Rubin et al., 2000, ) LP_cDNA
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]