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General Information
Name
RH_cDNA
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000016
Project
Created by
[BDGP: cDNA & EST FAQ](http://www.fruitfly.org/EST/faq.html)
Vector
Title
RH cap-trapped cDNA library of D. melanogaster, iso-1 strain, adult head.
Accessions
Overview
Description
Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.
Available from
Biosample Source
Overview
Sex
Other tissues studied
Cell component
Cell line
Key genes
Methods
Sample preparation
For the RH cDNA library, adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
Biosamples used (1)
Biosample
Type
Title
D. melanogaster, iso-1 strain, adult head, source for cDNA.
Reagent Details
Molecular Construct used
Protocol
RNA was polyA+ selected twice (RNA made by L. Hong). cDNA was synthesized by priming with the oligo(dT) primer adapter (5'-GAGAGAGAGAGGATCCAATACTGGAGAGTTTTTTTTTTTTTTTTVN-3'). The first strand was synthesized in presence of trehalose, which increases the full-length cDNA synthesis (Carninci, P. et al. 1998. Proc. Natl. Acad. Sci. U.S.A. 95(2): 520-524; Carninci, P. et al. 1999. Methods Enzymol. 303: 19-44). Full-length cDNA was selected with the biotinylated cap-trapper (Carninci, P. et al., Genomics 37: 327-336). A linker was then ligated to the single-strand cDNA following the published protocol (Shibata, Y., et al., Biotechniques, in press). Subsequently, the cDNA was normalized by using RoT=1.0 as published (Carninci, P., et al., Genome Res. 10: 1617-1630). Second strand cDNA was primed with the (5'-AGAGAGAGAGCTCGAGCTCTAATAAGGTGACACTATAGAACCA-3') primer. After restriction digestion of the hemimethylated cDNA with BamHI and XhoI, thecDNA was cloned in the lambda FLC-I vector. Subsequently, the library was bulk-excised into pFLC-I plasmid as described (Carninci, P., et al., Genomics, 2001, 77:79-90 ). cDNAs were transformed into DH5-alpha TonA strain.
Mode of Assay
Data analysis
Comments
Associated Data
Size
Associated features
52,475 Clone(s)
Files
Additional Information
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
RH
RH_cDNA
Riken head library
Name Synonyms
RH cap-trapped cDNA library of D. melanogaster, iso-1 strain, adult head.
Secondary FlyBase IDs
    References (1)