Open Close
General Information
Name
SD_cDNA
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000017
Project
Created by
[BDGP: cDNA & EST FAQ](http://www.fruitfly.org/EST/faq.html)
Vector
Title
SD cDNA library of D. melanogaster, Schneider's Line-2 cells.
Accessions
Overview
Description
Cloned cDNAs prepared from polyadenylated RNA isolated from cultured cells; cell line used was not derived the iso-1 sequenced strain.
Available from
Biosample Source
Overview
Strain
Stage
Sex
Tissue isolated
Other tissues studied
Cell component
Key genes
Methods
Sample preparation
For the SD cDNA library, Schneider L2 cells were collected.
Biosamples used (1)
Biosample
Type
Title
D. melanogaster, Schneider's Line-2 cells, source for cDNA.
Reagent Details
Molecular Construct used
Protocol
RNA was poly(A)+ selected once. oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~300,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).
Mode of Assay
Data analysis
Comments
Associated Data
Size
Associated features
18,885 Clone(s)
Files
Additional Information
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
SD
SD_cDNA
Name Synonyms
SD cDNA library of D. melanogaster, Schneider's Line-2 cells.
Secondary FlyBase IDs
    References (1)