Dataset UT_cDNA cDNA library

General Information
Name	UT_cDNA	Species	D. melanogaster
Dataset type	cDNA library	FlyBase ID	FBlc0000018
Source & Content
Consists of	cDNA clones in plasmid vector (Stapleton et al., 2003)
Created by	BDGP: cDNA & EST FAQ
Available from	Selected clones available from the DGRC; other sources listed at BDGP, Materials. BDGP: Materials DGRC: Vectors, cDNAs, ESTs
Strain
Stage & tissue
Stage Tissue/Position (including subcellular localization) Reference adult stage \| male testis (Stapleton et al., 2003)
Cell Line
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Members
Description	Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testis. The strain used was not the iso-1 sequenced strain. (Stapleton et al., 2003)
Parent collections
Component collection(s)
Number in collection
Comment on number in collection
Members
Experimental protocol
Vector	pOTB7 (Stapleton et al., 2003)
Sample preparation	A stem-cell-enriched testis library made from adult males overexpressing a UAS-unpaired transgene (P{UAS-os.C})in germ cells from the nos-vp16 Gal4 driver (P{GAL4::VP16-nos.UTR}). The UAS-unpaired transgenic flies were in a y,w genetic background and the nos-vp16 Gal4 driver were in a w background. Additionally there was a UAS-gfp transgene (possibly P{UAS-GFP.S65T}) recombined onto the nosGAL4 chromosome. RNA was isolated from hand dissected testes from 0-3 day old males. RNA kindly provided by the lab of Dr.Margaret Fuller. (Stapleton et al., 2003)
Collection preparation	cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha TonA. (Stapleton et al., 2003)
Mode of assay
Assay platform
Data analysis
Additional data
	More information is available under: UniGene: D. melanogaster UT
Associated files
Additional sites
Synonyms & Secondary IDs
Reported As
Symbol Synonym	UT UT_cDNA
Secondary FlyBase IDs

References ( 1 )
Unpublished	Stapleton et al., 2003, BDGP/HHMI UT Drosophila EST Project. BDGP/HHMI UT Drosophila EST Project. [FBrf0186645]