Dataset ESG01_cDNA cDNA library

General Information
Name	ESG01_cDNA	Species	D. melanogaster
Dataset type	cDNA library	FlyBase ID	FBlc0000020
Source & Content
Consists of	cDNA clones in plasmid vector (Gorski et al., 2003)
Created by	S.M. Gorski, Genome Sciences Centre, British Columbia Cancer Agency
Available from	S.M. Gorski, Genome Sciences Centre, British Columbia Cancer Agency
Strain	Oregon-R (Gorski et al., 2003)
Stage & tissue
Stage Tissue/Position (including subcellular localization) Reference pupal stage embryonic/larval salivary gland (Gorski et al., 2003)
Cell Line
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Description & Members
Description	Cloned cDNAs prepared from polyadenylated RNA isolated from pupal salivary glands. The strain used was not the iso-1 sequenced strain. (Gorski et al., 2003)
Parent collections
Component collection(s)
Number in collection
Comment on number in collection
Members
Experimental protocol
Vector	pSport1-Tag21 (Gorski et al., 2003)
Sample preparation	mRNA isolated from 500 pairs of hand-dissected salivary glands from mixed stage animals (16, 18, 20, 22, and 24 hrs after puparium formation at 18oC). (Gorski et al., 2003)
Collection preparation	Used to construct an oligo-dT-primed directional cDNA library (Vector: pSPORT1; Site_1: NotI; Site_2: Sal). (Gorski et al., 2003)
Mode of assay
Assay platform
Data analysis
Additional data
	More information is available under: UniGene: D. melanogaster ESG01
Associated files
Additional sites
Synonyms & Secondary IDs
Reported As
Symbol Synonym	ESG01 (Gorski et al., 2003, ) ESG01_cDNA
Secondary FlyBase IDs

References ( 1 )
Research paper	Gorski et al., 2003, Curr. Biol. 13(4): 358--363 A SAGE approach to discovery of genes involved in autophagic cell death. [FBrf0158811]