General Information
Name
DRSC_dsRNA
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000026
Project
Created by
Title
DRSC collection of dsRNA for RNAi in cell culture.
Accessions
    Overview
    Description
    Comprehensive collection of double-strand RNA fragments (1-2 dsRNAs per annotated gene), used for genome-wide RNAi-knockdown assays in cell culture.
    Available from
    Available for [on-site screening](https://fgr.hms.harvard.edu/drsc-20-genome-wide-screening-library). A limited number of amplicons can be requested as PCR-amplified DNA.
    Biosample Source
    Overview
    Strain
    Stage
    Sex
    Tissue isolated
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    Sample preparation
    Reagent Details
    Methods
    Protocol
    The DRSC RNAi screening library was constructed from genomic DNA amplified using Oregon-R strain. In initial collection, most amplicons were designed to FlyBase rel2 at R.Paro's lab (ZMBH Heidelberg). Primers were selected to target the open reading frames (ORF) of known and predicted genes. Regularly updated to accommodate newly identified genes and new gene models, and to replace amplicons with high potential for off-target effects.
    Mode of Assay
    Data analysis
    Comments
    Associated Data
    Size
    Associated features
    36,193 RNAi_reagent(s)
    Files
    Additional Information
    Synonyms and Secondary IDs (4)
    Reported As
    Symbol Synonym
    DRSC reagents
    DRSC_dsRNA
    Name Synonyms
    DRSC collection of dsRNA for RNAi in cell culture.
    Secondary FlyBase IDs
      References (3)