General Information
Name
DGRC-1
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000027
Project
    Created by
    Title
    A gene expression microarray consisting of spotted PCR-amplified genomic DNA corresponding to regions within annotated genes, DGRC.
    Overview
    Description
    Array of DNA fragments generated by PCR amplification; correspond to regions within postulated genes. Used for differential gene expression assays by hybridization to RNA. Technology type (GEO): spotted DNA/cDNA.
    Available from
    No longer available
    Biosample Source
    Overview
    Strain
    Stage
    Sex
    Tissue isolated
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    Sample preparation
    Reagent Details
    Methods
    Protocol
    DGRC-1 amplicon transcriptome microarrays are spotted with DNA fragments amplified from genomic DNA using gene-specific oligonucleotide primer pairs. The primers were made by Incyte Genomics and were generously donated to the DGRC by Incyte Genomics and Brian Oliver (NIDDK, National Institutes of Health). The resulting microarray consists of 15,552 spots corresponding to 88% of the genes in the Drosophila melanogaster annotation release 4.3.
    Mode of Assay
    Data analysis
    Comments
    Genomic location data received from James Costello on May 11 2006 as a curated list of coordinates based on BLAST analysis of predicted amplicons against Dmel release 4; migrated to release 5 by FlyBase.
    Associated Data
    Size
    Files
    Additional Information
    Synonyms and Secondary IDs (4)
    Reported As
    Symbol Synonym
    DGRC-1
    DGRC-D. melanogaster-DGRC1-15552
    GEO Platform GPL3830
    Name Synonyms
    A gene expression microarray consisting of spotted PCR-amplified genomic DNA corresponding to regions within annotated genes, DGRC.
    Secondary FlyBase IDs
      References (1)