Array of long synthesized oligonucleotides that correspond to regions within postulated genes. Used for differential gene expression assays by hybridization to RNA. Technology type (GEO): spotted oligonucleotide.
DGRC-2 transcriptome microarrays are spotted with gene-specific Drosophila melanogaster 70 N long oligonucleotides, prepared in conjunction with INDAC. The oligos were designed according to release 4.1 of the genome annotation using a custom implementation of OligoArray2. Oligos selected have minimal sequence similarity to other genes, size range between 69-65 nucleotides with a minimal Tm window, and locations biased towards the 3'-ends of transcripts. The resulting 70-mer oligonucleotide transcriptome microarray contains 15,158 unique oligo's corresponding to 93% of the genes in the Drosophila melanogaster annotation v. 4.3.
Genomic location data received from James Costello on May 13 2006 as a curated list of coordinates based on BLAST analysis of oligonucleotides against Dmel release 4; migrated to release 5 by FlyBase.
