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General Information
Name
DGRC-2
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000028
Project
    Created by
    Vector
      Title
      A gene expression microarray consisting of oligonucleotides corresponding to regions within annotated genes, DGRC.
      Accessions
      Overview
      Description

      Array of long synthesized oligonucleotides that correspond to regions within postulated genes. Used for differential gene expression assays by hybridization to RNA. Technology type (GEO): spotted oligonucleotide.

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      No longer available
      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Protocol

      DGRC-2 transcriptome microarrays are spotted with gene-specific Drosophila melanogaster 70 N long oligonucleotides, prepared in conjunction with INDAC. The oligos were designed according to release 4.1 of the genome annotation using a custom implementation of OligoArray2. Oligos selected have minimal sequence similarity to other genes, size range between 69-65 nucleotides with a minimal Tm window, and locations biased towards the 3'-ends of transcripts. The resulting 70-mer oligonucleotide transcriptome microarray contains 15,158 unique oligo's corresponding to 93% of the genes in the Drosophila melanogaster annotation v. 4.3.

      Mode of Assay
      Data analysis
      Comments

      Genomic location data received from James Costello on May 13 2006 as a curated list of coordinates based on BLAST analysis of oligonucleotides against Dmel release 4; migrated to release 5 by FlyBase.

      Associated Data
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      Files
      Additional Information
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      DGRC-2
      DGRC-D. melanogaster-DGRC2-17328-v1
      GEO Platform GPL3880
      Name Synonyms
      A gene expression microarray consisting of oligonucleotides corresponding to regions within annotated genes, DGRC.
      Secondary FlyBase IDs
        References (1)