General Information
Name
VDRC-GD
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000041
Project
Created by
Title
A set of transgenic RNAi constructs for expression of dsRNA under UAS control, VDRC first generation.
Accessions
    Overview
    Description
    Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.
    NOTE: Dataset members correspond to the amplicon sequence features; these can be used to retrieve associated constructs and stocks.
    Available from
    Biosample Source
    Overview
    Strain
    Stage
    Sex
    Tissue isolated
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    Sample preparation
    Reagent Details
    Methods
    Molecular Construct used
    Protocol
    A collection of over 13,200 RNAi transgenes (over 22,200 lines), each designed to target a single protein-coding gene; each construct contains short gene fragments cloned as inverted repeats that are expressed using the binary GAL4/UAS system. It is estimated that 88% of predicted protein-coding genes are targeted.
    Mode of Assay
    Data analysis
    A specificity score (Q) was calculated for each construct, such that if Q is < 0.5, off-target effects are probable and if Q > 0.8, off-target effects are improbable.
    Comments
    A dsRNA transgene may affect more than the targeted gene if (1) the target gene is a member of an paralogous gene family or (2) there are appreciable off-target effects. By conservative criteria, approximately 5% of the constructs in the collection may have experimentally significant off-target effects.
    Associated Data
    Size
    Associated features
    15,399 RNAi_reagent(s)
    Additional Information
    [More information available at VDRC](http://stockcenter.vdrc.at/control/library_rnai)
    Synonyms and Secondary IDs (3)
    Reported As
    Symbol Synonym
    VDRC-GD
    Name Synonyms
    A set of transgenic RNAi constructs for expression of dsRNA under UAS control, VDRC first generation.
    Secondary FlyBase IDs
      References (6)