Dataset VDRC-1

General Information
Name	VDRC-1	Species	D. melanogaster
Dataset type	dsRNA construct collection	FlyBase ID	FBlc0000041
Source & Content
Consists of	Fly stocks carrying dsRNA transgenic constructs. (Dietzl et al., 2007)
Created by	VDRC
Available from	VDRC
Strain
Stage & tissue

Cell Line
Recent Updates
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FB2013_03
FB2013_02
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Description & Members
Description	Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene. (Dietzl et al., 2007)
Parent collections
Component collection(s)
Number in collection
Comment on number in collection
Members
Experimental protocol
Vector	pP{MF3} (Dietzl et al., 2007)
Sample preparation
Collection preparation	A collection of over 13,200 RNAi transgenes (over 22,200 lines), each designed to target a single protein-coding gene; each construct contains short gene fragments cloned as inverted repeats that are expressed using the binary GAL4/UAS system. It is estimated that 88% of predicted protein-coding genes are targeted. (Dietzl et al., 2007)
Mode of assay
Assay platform
Data analysis	"Possible off-target effects." (Dickson et al., 2007.7.18) "Possible off-target effects (low probability)." (Dickson et al., 2007.7.18) "Probable off-target effects." (Dickson et al., 2007.7.18) "Targets multiple paralogous genes." (Dickson et al., 2007.7.18) Descriptive statements about off-target and multi-target information correspond to the following parameter ranges: (Dickson et al., 2007.7.18) For multiple on-target genes (M = number of on-target genes) (Dickson et al., 2007.7.18) For specificity (off-target effects; Q = Specificity score) (Dickson et al., 2007.7.18) If 0.5 < Q =<0.8 (Dickson et al., 2007.7.18) If 0.8 < Q < 1 (Dickson et al., 2007.7.18) If M = 1, no comment (Dickson et al., 2007.7.18) If M > 1 AND Q > 0.5 (Dickson et al., 2007.7.18) If Q = 1, no comment (Dickson et al., 2007.7.18) If Q =< 0.5 (Dickson et al., 2007.7.18)
Additional data
	Detailed data concerning off-target parameters are included in the bulk data files provided to FlyBase (on FlyBase ftp site) and in FBrf0202579: number of on/off target genes; number of on/off target 19-mers; number of CAN/CAR repeats; specificity score. (Dickson et al., 2007.7.18) More information is available under: Dickson.2007.8.27-1.Vienna_constructs.xls Dickson.2007.8.27-2.Vienna_insertions.xls
Associated files	Dickson.2007.8.27-1.Vienna_constructs.xls (Dickson et al., 2007.7.18) Dickson.2007.8.27-2.Vienna_insertions.xls (Dickson et al., 2007.7.18)
Additional sites
Comments
	A dsRNA transgene may affect more than the targeted gene if (1) the target gene is a member of an paralogous gene family or (2) there are appreciable off-target effects. By conservative criteria, approximately 5% of the constructs in the collection may have experimentally significant off-target effects. (Dietzl et al., 2007)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	VDRC-1
Secondary FlyBase IDs

References ( 3 )
Research paper	Dietzl et al., 2007, Nature 448(7150): 151--156 A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. [FBrf0200691]
Supplementary material	Dietzl et al., 2007, Nature 448(7150): Supplementary Table 1. [FBrf0202579]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]