Dataset TTT_DIG_cDNA

General Information
Name	TTT_DIG_cDNA	Species	D. melanogaster
Dataset type	cDNA sequence	FlyBase ID	FBlc0000042
Source & Content
Consists of	EST sequences (uncloned) (Torres et al., 2008)
Created by	Tatiana T. Torres, Veterinarmedizinische Universitat Wien
Available from	Unavailable as reagents (not cloned).
Strain	iso-1 (Torres et al., 2008)
Stage & tissue
Stage Tissue/Position (including subcellular localization) Reference adult stage \| female organism (Torres et al., 2008)
Cell Line
Recent Updates
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FB2013_03
FB2013_02
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Description & Members
Description	Sequence of 3' ends of cDNAs prepared from total RNA from adult females. (Torres et al., 2008)
Parent collections
Component collection(s)
Number in collection
Comment on number in collection
Members
Experimental protocol
Vector
Sample preparation	Total RNA was extracted from D. melanogaster adult virgin females aged 3-7 days. (Torres et al., 2008)
Collection preparation	Double-stranded cDNA was prepared from total RNA, using an oligo-dT primer for first-strand synthesis. Preparations were digested separately with Mbo I, Nla III and Tai I, and 3' fragments recovered. The 3' fragments produced by each restriction enzyme were pooled before sequencing. (Torres et al., 2008)
Mode of assay	cDNA was characterized by high-throughput sequencing. (Torres et al., 2008)
Assay platform	454 GS 20 (Torres et al., 2008)
Data analysis
Additional data
	More information is available under: GEO: GPL9772 UniGene: D. melanogaster Torres digested cDNA
Associated files
Additional sites
Synonyms & Secondary IDs
Reported As
Symbol Synonym	TTT_DIG (Torres et al., 2008) TTT_DIG_cDNA
Secondary FlyBase IDs

References ( 1 )
Research paper	Torres et al., 2008, Genome Res. 18(1): 172--177 Gene expression profiling by massively parallel sequencing. [FBrf0202153]