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General Information
Name
TTT_DIG_cDNA
Species
D. melanogaster
Assay type
FlyBase ID
FBlc0000042
Project
Data Provider
Tatiana T. Torres, Veterinarmedizinische Universitat Wien
Title
RNA-Seq of D. melanogaster, iso-1 strain, adult virgin female, 3' ends of polyadenylated transcripts.
Accessions
Biosample Source
Overview
Tissue isolated
Other tissues studied
Cell component
Cell line
Key genes
Methods
Sample preparation
Biosamples used (1)
Biosample
Type
Title
D. melanogaster, iso-1 strain, adult virgin female, source for cDNA.
Assay Details
Key genes
Protocol

Total RNA was isolated and double-stranded cDNA was prepared using an oligo-dT primer for first-strand synthesis. Preparations were digested separately with Mbo I, Nla III and Tai I, and 3' fragments recovered. The 3' fragments produced by each restriction enzyme were pooled before sequencing.

Mode of Assay

cDNA was characterized by high-throughput sequencing using the "454 GS 20" platform.

Comments
Associated Data
Size
Associated features
8,053 Clone(s)
Files
Additional Information
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
TTT_DIG_cDNA
Name Synonyms
RNA-Seq of D. melanogaster, iso-1 strain, adult virgin female, 3' ends of polyadenylated transcripts.
Secondary FlyBase IDs
    References (1)