FB2022_03, released June 9, 2022

Assay: TTT_NEB_cDNA

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General Information
Name
TTT_NEB_cDNA
Species
D. melanogaster
Assay type
RNA-Seq
FlyBase ID
FBlc0000043
Project
Data Provider
Tatiana T. Torres, Veterinarmedizinische Universitat Wien
Title
RNA-Seq of D. melanogaster, iso-1 strain, adult virgin female, 3' ends of polyadenylated transcripts.
Accessions
Biosample Source
Overview
Strain
iso-1
(Torres et al., 2008)
Stage
adult stage
(Torres et al., 2008)
Sex
virgin female
(Torres et al., 2008)
Tissue isolated
organism
(Torres et al., 2008)
Other tissues studied
Cell component
Cell line
Key genes
Methods
Sample preparation
Biosamples used (1)
Biosample
Type
Title
BS_TTT_NEB_cDNA
whole organism
D. melanogaster, iso-1 strain, adult virgin female, source for cDNA.
Assay Details
Methods
oligo-dT priming
(Torres et al., 2008)
454 sequencing
(Torres et al., 2008)
Key genes
Protocol

Total RNA was isolated and double-stranded cDNA was prepared using an oligo-dT primer for first-strand synthesis. Preparations were nebulized, 3' fragments recovered, and sequenced.

(Torres et al., 2008)
Mode of Assay

cDNA was characterized by high-throughput sequencing using the "454 GS 20" platform.

(Torres et al., 2008)
Comments
Associated Data
Size
Associated features
9,345 Clone(s)
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Files
Additional Information
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
TTT_NEB
(Torres et al., 2008)
TTT_NEB_cDNA
Name Synonyms
RNA-Seq of D. melanogaster, iso-1 strain, adult virgin female, 3' ends of polyadenylated transcripts.
(Torres et al., 2008)
Secondary FlyBase IDs
    References (1)