General Information
Name
TRiP-1
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000048
Project
Created by
Title
A set of transgenic RNAi constructs for expression of dsRNA under UAS control, TRiP first generation (pVALIUM10).
Accessions
    Overview
    Description
    Collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene; use a targeted insertion site.
    A collection of first generation of UAS-driven long RNAi hairpin constructs cloned into derivatives of the VALIUM vector (Vermilion-AttB-Loxp-Intron-UAS-MCS) and targeted into the genome by the phiC31-mediated integration.
    NOTE: Dataset members correspond to the amplicon sequence features; these can be used to retrieve associated constructs and stocks.
    Biosample Source
    Overview
    Strain
    Stage
    Sex
    Tissue isolated
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    Sample preparation
    Reagent Details
    Methods
    Molecular Construct used
    Transgenic Construct used
    Protocol
    The approach used by the TRiP is to generate transgenic animals with an RNAi hairpin under UAS-GAL4 control. The hairpin-containing transgenes are inserted via site-specific recombination into genomic loci known to be optimal for expression. This specific collection was constructed in the pVALIUM10 vector and inserted into the P{CaryP}attP2 target element.
    Primers for hairpin constructs were designed using the DRSC amplicon design tool SnapDragon (http://www.flyrnai.org/cgi-bin/RNAi_find_primers.pl). First, regions of 400-600bp that are free of EcoRI and XbaI restriction sites, and free of 19bp predicted off-target sites. If this was not possible, then regions without 20 or 21 bp matches to other genes were used. Primers were used to PCR amplify target DNA. Double-stranded RNA (dsRNA) hairpin constructs were cloned into the VALIUM10 vector, which allows for phiC31 targeted integration into the genome and Gal4-UAS inducible expression. Targeted transgene integration reduces the variability in hairpin expression resulting from random transgene integration.
    Mode of Assay
    Data analysis
    Comments
    "TRiP" stands for Transgenic RNAi Project
    A critical feature of TRiP-1 (VALIUM10-based) constructs, compared to TRiP-2 (VALIUM1-based) constructs, is the presence of gypsy insulator sequences that boost dramatically the level of knockdown.
    Associated Data
    Size
    Associated features
    1,799 RNAi_reagent(s)
    Files
    Additional Information
    [TRiP Approach - 1st generation](http://www.flyrnai.org/TRiP-TTR-GEN1.html)
    Synonyms and Secondary IDs (4)
    Reported As
    Symbol Synonym
    dsRNAs expressed from VALIUM10
    Name Synonyms
    A set of transgenic RNAi constructs for expression of dsRNA under UAS control, TRiP first generation (pVALIUM10).
    Secondary FlyBase IDs
      References (7)