Individual PCR products were pooled to create a molar-normalized mixtures of 1,440 to 2,677 products using a Tecan Genesis 2 robot. The sample pools were then concentrated to approximatley 50 ng/ul. Normalized pools of RACE products were created and either cloned and sequenced with an ABI3730 or by high-throughput sequencing using the "454 GS FLX" platform. Vector sequences were removed and the RNA adapter sequence was used to determine the orientation of the clone and was removed from the sequence. Each sequence represents a potential transcription start site and is oriented along the direction of transcription.