General Information
Name
VDRC-KK
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000055
Project
Created by
Title
A set of transgenic RNAi constructs for expression of dsRNA under UAS control, VDRC second generation.
Accessions
    Overview
    Description
    Collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene; use a targeted insertion site.
    NOTE: Dataset members correspond to the amplicon sequence features; these can be used to retrieve associated constructs and stocks.
    Available from
    Biosample Source
    Overview
    Strain
    Stage
    Sex
    Tissue isolated
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    Sample preparation
    Reagent Details
    Methods
    Molecular Construct used
    Transgenic Construct used
    Protocol
    The phiC31 RNAi library (KK) was created using the phiC31 integrase system to target RNAi transgenes to a specific landing site on the second chromosome. This insertion site (P{attP,y+,w3'}VIE-260B) was selected based on its low level of basal expression and consistent high level of GAL4-dependent expression across a range of different tissues. The RNAi transgenes consist of inverted repeats that were designed to reduce the risk of off-target effects.
    The host strain used for transgene integration actually has two landing sites: the annotated site in the 5' UTR of tio at R6:2L:22 ,019,296 (P{attP,y+,w3'}VIE-260B), and a previously non-annotated site at R6:2L:9437482 (P{attP,y+,w3'}VIE-260B-2). The previously non-annotated site seems to be the main integration site, occupied in all 39 lines tested. The originally annotated site was occupied in only 9 of 39 lines tested.
    Mode of Assay
    Data analysis
    Comments
    Of 39 VDRC KK lines tested, the 9 lines with integrations at the originally annotated landing site ( 2L:22019296 ) were all associated with a non-inflating wing phenotype using the P{GawB}elavC155 driver, and pupal lethality using the P{Act5C-GAL4}17bFO1 driver. This is presumably due to overexpression of the nearby tio gene.
    Researchers are encouraged to validate results using KK lines. PCR diagnostics can be used to identify strains with integration at the problematic landing site, and recombination can be used to 'clean' lines with problematic integrations.
    Associated Data
    Size
    Associated features
    10,748 RNAi_reagent(s)
    Files
    Additional Information
    [More information available at VDRC](http://stockcenter.vdrc.at/control/library_rnai)
    Synonyms and Secondary IDs (6)
    Reported As
    Symbol Synonym
    KK
    VDRC KK collection
    VDRC-KK
    phiC31 RNAi library (KK)
    Name Synonyms
    A set of transgenic RNAi constructs for expression of dsRNA under UAS control, VDRC second generation.
    Secondary FlyBase IDs
      References (6)