Dataset DPiM_cDNA_bait_cDNA cDNA library

General Information
Name	DPiM_cDNA_bait_cDNA	Species	D. melanogaster
Dataset type	cDNA library	FlyBase ID	FBlc0000056
Source & Content
Consists of	cDNA bait clones (tagged, inducible) (Mintseris et al., 2009.11.23)
Created by	DPiM: Drosophila Protein interaction Map
Available from
Strain
Stage & tissue

Cell Line
Recent Updates
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FB2013_03
FB2013_02
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Description & Members
Description	Full-length open reading frame fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter. (Mintseris et al., 2009.11.23)
Parent collections
Component collection(s)
Number in collection
Comment on number in collection
Members
Experimental protocol
Vector	pMK33-C-TAP-FLAG-HA-BD (Mintseris et al., 2009.11.23)
Sample preparation
Collection preparation	A cDNA sequence encoding each bait molecule was cloned into the pMK33-C-TAP-FLAG-HA-BD vector as a full-length open reading frame, fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter. cDNA sequences are derived from previously characterized and publicly available clones created by the Berkeley Drosophila Genome Project; all inserts are full-length sequence-validated. (Mintseris et al., 2009.11.23)
Mode of assay
Assay platform
Data analysis
Additional data
	More information is available under: DPiM: Drosophila Protein interaction Map
Associated files
Additional sites
Synonyms & Secondary IDs
Reported As
Symbol Synonym	DPiM_cDNA_bait DPiM_cDNA_bait_cDNA
Secondary FlyBase IDs

References ( 2 )
Research paper	Guruharsha et al., 2011, Cell 147(3): 690--703 A Protein Complex Network of Drosophila melanogaster. [FBrf0216491]
Personal communication to FlyBase	Mintseris et al., 2009.11.23, DPiM: the Drosophila Protein Interaction Mapping project DPiM: the Drosophila Protein Interaction Mapping project [FBrf0209452]