Dataset BCM_1_P_junctions

General Information
Name	BCM_1_P_junctions	Species	D. melanogaster
Dataset type	RNA-Seq transcriptome junctions	FlyBase ID	FBlc0000082
Source & Content
Consists of	cDNA short sequencing reads from high-throughput sequencing, junctions only. (Daines et al., 2010.2.8)
Created by
Available from
Strain	Canton-S (Daines et al., 2010.2.8)
Stage & tissue
Stage Tissue/Position (including subcellular localization) Reference pupal stage organism (Daines et al., 2010.2.8) Comment:aged 1 to 5 days after collection as third instar larvae
Cell Line
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Members
Description	Short sequencing reads of cDNA prepared from polyadenylated RNA isolated from pupae ranging from prepupal to pharate adult stages. RNA junctions (corresponding to introns) identified and characterized (presented in GBrowse and in RNA junction reports). (Daines et al., 2010.2.8)
Parent collections	BCM_1_RNAseq_junctions
Component collection(s)
Number in collection
Comment on number in collection
Members
Experimental protocol
Vector
Sample preparation	See report for BCM_1_RNAseq. (Daines et al., 2010.2.8)
Collection preparation	RNA recovered from 5 pupal collections representing successive stages (daily collections, 1-5 days after third larval instar) was mixed in equimolar amounts. See report for BCM_1_RNAseq for additional information. (Daines et al., 2010.2.8)
Mode of assay	Platform configuration, 75bp paired-end reads. This library was sequenced in replicate lanes. See report for BCM_1_RNAseq for additional information. (Daines et al., 2010.2.8)
Assay platform	See report for BCM_1_RNAseq. (Daines et al., 2010.2.8)
Data analysis	See report for BCM_1_RNAseq. (Daines et al., 2010.2.8)
Additional data

Associated files
Additional sites
Synonyms & Secondary IDs
Reported As
Symbol Synonym	BCM_1_P_junctions Pupa (Daines et al., 2011) Pupa (P) (Daines et al., 2010.2.8)
Secondary FlyBase IDs

References ( 2 )
Research paper	Daines et al., 2011, Genome Res. 21(2): 315--324 The Drosophila melanogaster transcriptome by paired-end RNA sequencing. [FBrf0213064]
Personal communication to FlyBase	Daines et al., 2010.2.8, Drosophila melanogaster mRNA high-throughput sequencing Drosophila melanogaster mRNA high-throughput sequencing [FBrf0210193]