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General Information
Name
mE_S2-DRSC_polyA
Species
D. melanogaster
Result type
FlyBase ID
FBlc0000120
Project
Data Provider
[modENCODE: Drosophila transcriptome](http://www.modencode.org/celniker/)
Title
RNA-Seq coverage profile of D. melanogaster, S2-DRSC cell line, modENCODE, unstranded.
Status
Current
Accessions
    Biosample Source
    Overview
    Strain
    Stage
    Sex
    Tissue isolated
    Other tissues studied
    Cell component
    Key genes
    Methods
    Sample preparation

    Cells were grown in Schneider's (FBrf0024118) supplemented with 10% fetal calf serum.

    Cells were maintained between 2E6 and 1E7 cells/mL, and were harvested at about 5E6 cells/mL.

    Biosamples analyzed by this result (1)
    Biosample
    Type
    Title
    D. melanogaster, S2-DRSC cell line, source for RNA.
    Data Analyzed
    Key genes
    Protocol

    Total RNA was isolated using Trizol, then Qiagen RNeasy spin columns. mRNA was isolated using Oligotex kit, then fragmented using divalent cations under elevated temperature. First and second strand cDNA synthesis was primed with random hexamers. The cDNA fragments were end-repaired using T4 DNA polymerase and Klenow DNA polymerase, and phosphorylated at their 5' ends with T4 polynucleotide kinase. After adding A bases to the 3' end of the DNA fragments, Illumina adaptor oligonucleotides were ligated to the ends and ~ 300 bp fragments were isolated from an agarose gel, enriched by PCR amplification, and gel-purified again.

    Mode of Assay

    Read length (bases):37

    Read length (bases):76

    The samples were quantitated using a Nanodrop, and loaded onto a flow cell for cluster generation and sequenced on an Illumina Genome Analyzer II using either single read or paired end protocols (Illumina).

    Raw Data Analyzed (1)
    Assay / Reagent collection
    Type
    Title
    RNA-Seq of D. melanogaster, S2-DRSC cell line, modENCODE, unstranded.
    Processed Data Analyzed (0)
    Result
    Type
    Title
    Analysis
    Reference Genome
    Reference Annotation
    Data analysis
    Comments
    Associated Data
    Size
    Files
    Additional Information
    Synonyms and Secondary IDs (5)
    Reported As
    Symbol Synonym
    S2-DRSC (polyA RNA), Bowtie mapper
    S2-DRSC_polyA, Bowtie mapper
    cell.2_S2-DRSC_polyA
    mE_S2-DRSC_polyA
    Name Synonyms
    RNA-Seq coverage profile of D. melanogaster, S2-DRSC cell line, modENCODE, unstranded.
    Secondary FlyBase IDs
      References (2)