Dataset mE_Early_Replication_Origins_cells

General Information
Name	mE_Early_Replication_Origins_cells	Species	D. melanogaster
Dataset type	genomic sequence feature	FlyBase ID	FBlc0000188
Source & Content
Consists of	Genomic sequences identified by integrative analysis of several ChIP data sets. (Eaton et al., 2010.12.17)
Created by	modENCODE: Origins of Replication Group
Available from	Not available as reagents.
Strain
Stage & tissue

Cell Line	Kc167, S2-DRSC, ML-DmBG3-c2
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Members
Description	Genomic sequences identified as early activating origins of replication in at least one of three cell lines analyzed; a synthesis of component data sets. (Eaton et al., 2010.12.17)
Parent collections
Component collection(s)	mE_Early_Replication_Origins_Kc167 mE_Early_Replication_Origins_ML-DmBG3-c2 mE_Early_Replication_Origins_S2-DRSC
Number in collection
Comment on number in collection
Members
Experimental protocol
Vector
Sample preparation	BrdU labeling was in the presence of hydroxyurea, a potent inhibitor of nucleotide biosynthesis, resulting in stalled replication forks. Thus, only those sequences immediately adjacent to early activating origins or replication will incorporate BrdU. (Eaton et al., 2011) See component data set reports for details. (Eaton et al., 2010.12.17)
Collection preparation	See component data set reports for details. (Eaton et al., 2010.12.17)
Mode of assay	See component data set reports for details. (Eaton et al., 2010.12.17)
Assay platform	See component data set reports for details. (Eaton et al., 2010.12.17)
Data analysis	The union of early activating origins of replication from all 3 cell lines was taken to generate a set of meta-peaks; each meta-peak represents a contiguous region covered by an early activating origin of replication present in at least one cell line. (Eaton et al., 2010.12.17)
Additional data
	More information is available under: GEO: GSE27981 modMine: modENCODE_3441
Associated files
Additional sites
Comments
	modENCODE Origins of Replication (Eaton et al., 2010.12.17)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	early origin meta-peaks (Eaton et al., 2010.12.17) mE_Early_Replication_Origins_cells
Secondary FlyBase IDs

References ( 2 )
Research paper	Eaton et al., 2011, Genome Res. 21(2): 164--174 Chromatin signatures of the Drosophila replication program. [FBrf0213065]
Personal communication to FlyBase	Eaton et al., 2010.12.17, Incorporation of modENCODE Replication Origins Data into FlyBase. Incorporation of modENCODE Replication Origins Data into FlyBase. [FBrf0212681]