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General Information
Name
mE_Orc2_ChIP-Seq_cells
Species
D. melanogaster
Result type
FlyBase ID
FBlc0000189
Project
Data Provider
[modENCODE: Drosophila replication origins](http://www.modencode.org/macalpine/)
Title
Orc2 binding sites, analysis of ChIP-Seq in three cell lines (meta-peaks).
Status
Current
Accessions
Biosample Source
Overview
Strain
Stage
Sex
Tissue isolated
Other tissues studied
Cell component
Key genes
Methods
Sample preparation

Cells were grown to a density of 3E6 cells/ml, and 1E8 cells from asynchronous cultures were used for each experiment.

Biosamples analyzed by this result (3)
Biosample
Type
Title
D. melanogaster, Kc167 cell line, source for Orc2 ChIP.
D. melanogaster, ML-DmBG3-c2 cell line, source for Orc2 ChIP.
D. melanogaster, S2-DRSC cell line, source for Orc2 ChIP.
Data Analyzed
Key genes
(bait_protein)
Protocol
Mode of Assay
Raw Data Analyzed (3)
Assay / Reagent collection
Type
Title
ChIP-Seq of Orc2 in D. melanogaster, S2-DRSC cell line.
ChIP-Seq of Orc2 in D. melanogaster, ML-DmBG3-c2 cell line.
ChIP-Seq of Orc2 in D. melanogaster, Kc167 cell line.
Processed Data Analyzed (0)
Result
Type
Title
Analysis
Methods
Reference Genome
Reference Annotation
Data analysis

Reads were mapped to the genome assembly using MAQ, allowing for up to 3 mismatches (quality score of at least 35). PeakSeq was used to make peak calls with a maximum q-val of 0.05 for each replicate. Replicates were combined by intersection: overlapping peak calls were considered verified and were reduced in a per-nucleotide union. The union of Orc2 binding sites from all 3 cell lines was taken to generate a set of meta-peaks; each meta-peak represents a contiguous region covered by an early activating origin of replication present in at least one cell line. In total, 7246 meta-peaks were called, 2395 common to all three cell lines.

Comments
Associated Data
Size
Associated features
7,246 origin_of_replication(s)
Files
Additional Information
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
ORC meta-peaks
mE_Orc2_ChIP-Seq_cells
Name Synonyms
Orc2 binding sites, analysis of ChIP-Seq in three cell lines (meta-peaks).
Secondary FlyBase IDs
    References (3)