Dataset GMR_Brain_exp_1

General Information
Name	GMR_Brain_exp_1	Species	D. melanogaster
Dataset type	construct collection	FlyBase ID	FBlc0000201
Source & Content
Consists of	Fly stocks carrying GAL4 transgenic constructs designed to be expressed in adult brain. (Pfeiffer et al., 2008)
Created by
Available from	Bloomington Drosophila Stock Center
Strain	iso-1 (Pfeiffer et al., 2008)
Stage & tissue

Cell Line
Recent Updates
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FB2013_03
FB2013_02
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Description & Members
Description	Collection of stocks carrying GAL4 transgenes fused to defined putative enhancer fragments from selected D. melanogaster genes; a targeted insertion site is used. The goal is to establish a collection of enhancers driving expression in small subsets of cells in adult brain. (Pfeiffer et al., 2008)
Parent collections
Component collection(s)
Number in collection
Comment on number in collection
Members
Experimental protocol
Vector	P{CaryP} (Pfeiffer et al., 2008) pBPGUw (Pfeiffer et al., 2008) pBPGw (Pfeiffer et al., 2008)
Sample preparation	925 genes for which available expression data or predicted function implied expression in a subset of cells in the adult brain were selected. Spanning the flanking upstream and downstream intergenic regions of these genes, as well as any of their introns larger than 300 bp, regions were selected for testing that averaged 3 kb in length and overlapped (in regions that could not be covered by a single fragment) by approximately 1 kb. (Pfeiffer et al., 2008)
Collection preparation	The fragment of genomic DNA to be tested for enhancer activity was generated by PCR, cloned into a Gateway donor vector, and verified by DNA sequencing. Site-specific recombination was used to transfer the fragment into the integration vector pBPGUw or pBPGw. Constructs in pBPGUw use a Drosophila synthetic core promoter (DSCP). Constructs in pBPGw use the putative endogenous promoter of the selected gene. Constructs were inserted into the P{CaryP}attP2 target element by phiC31 site-specific integration. (Pfeiffer et al., 2008)
Mode of assay	Expression of GAL4 was detected either directly by in situ hybridization to the GAL4 mRNA or by assaying expression of a UAS-GFP fusion gene. (Pfeiffer et al., 2008)
Assay platform
Data analysis	Eventual goal is to select lines directing expression in small subsets of cells (typically 10-200 cells) in the adult brain. (Pfeiffer et al., 2008)
Additional data

Associated files
Additional sites
Synonyms & Secondary IDs
Reported As
Symbol Synonym	GMR_Brain_exp_1
Secondary FlyBase IDs

References ( 1 )
Research paper	Pfeiffer et al., 2008, Proc. Natl. Acad. Sci. U.S.A. 105(28): 9715--9720 Tools for neuroanatomy and neurogenetics in Drosophila. [FBrf0205679]