General Information
Name
BCM_1_RNAseq_junctions
Species
D. melanogaster
Project type
FlyBase ID
FBlc0000204
Parent Project
Data Provider
Title
Exon junction identification from RNA-Seq assays of D. melanogaster, Canton-S strain, 10 developmental stages.
Accessions
    Overview
    Description
    Umbrella record for 12 collections that differ by developmental stage, from embryos to aged adults. RNA junctions identified and characterized (presented in GBrowse and in RNA junction reports).
    Project attributes
    Reagent type
    Key genes
    GO term(s)
    SO term(s)
    Details
    Sample preparation
    Protocol
    Mode of Assay
    Data analysis
    Comments
    A small number of junctions predicting very small and very long introns may be mispredictions and should be interpreted with care; these are currently being re-examined.
    For ~1900 predicted junctions, the supporting evidence came from a methodology (Wang L, Xi Y, Yu J, Dong L, Yen L, et al. 2010 A Statistical Method for the Detection of Alternative Splicing Using RNA-Seq. PLoS ONE 5(1): e8529. doi:10.1371/journal.pone.0008529) that did not report number of supporting junction-spanning reads. Each of these ~1900 predicted junctions was supported by at least two reads, and so we have noted these by having a total junction-spanning read count of ">1". However, we do not know which developmental stages these supporting reads were in, and so all developmental stages are reported as "0".
    Files
    Additional Information
    Synonyms and Secondary IDs (2)
    Reported As
    Symbol Synonym
    BCM_1_RNAseq_junctions
    Name Synonyms
    Exon junction identification from RNA-Seq assays of D. melanogaster, Canton-S strain, 10 developmental stages.
    Secondary FlyBase IDs
      References (3)