Dataset mE_mRNA_L3_EtOH_5

General Information
Name	mE_mRNA_L3_EtOH_5	Species	D. melanogaster
Dataset type	RNA-Seq transcriptome coverage	FlyBase ID	FBlc0000255
Source & Content
Consists of	cDNA short sequencing reads from high-throughput sequencing. (Graveley et al., 2011.4.14)
Created by
Available from
Strain	Oregon-R-modENCODE (Graveley et al., 2011.4.14)
Stage & tissue
Stage Tissue/Position (including subcellular localization) Reference third instar larval stage organism (Graveley et al., 2011.4.14) Comment:treatment: ethanol exposure (5%)
Cell Line
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Members
Description	Short sequencing reads of cDNA prepared from polyadenylated RNA isolated from third instar larvae exposed to ethanol (5% for 3 hours). Transcriptome represented as frequency of strand-specific reads along genome (see GBrowse presentation; data in wiggle format); this collection is composed of uniquely aligned reads. (Graveley et al., 2011.4.14)
Parent collections	modENCODE_mRNA-Seq_treatments
Component collection(s)
Number in collection	157,058,163 (Graveley et al., 2011.4.14)
Comment on number in collection	Number of unique reads. (Graveley et al., 2011.4.14)
Members
Experimental protocol
Vector
Sample preparation	Third instar larvae were cultured on food supplemented with 5% EtOH for 3 hours; 2.7% lethality was observed. Survivors were flash frozen in liquid nitrogen and stored at -80 degrees C prior to RNA preparations. See report for modENCODE_mRNA-Seq_treatments for information on RNA isolation. (Graveley et al., 2011.4.14)
Collection preparation	See report for modENCODE_mRNA-Seq_treatments. (Graveley et al., 2011.4.14)
Mode of assay	Protocol: strand-specific (Graveley et al., 2011.4.14) Read length (bases):76 (Graveley et al., 2011.4.14) See report for modENCODE_mRNA-Seq_treatments. (Graveley et al., 2011.4.14)
Assay platform	See report for modENCODE_mRNA-Seq_treatments. (Graveley et al., 2011.4.14)
Data analysis	See report for modENCODE_mRNA-Seq_treatments. (Graveley et al., 2011.4.14)
Additional data

Associated files
Additional sites
Synonyms & Secondary IDs
Reported As
Symbol Synonym	5% EtOH Third Instar larvae (Graveley et al., 2011.4.14) EtOH_5_L3 (Graveley et al., 2011.4.14) mE_mRNA_L3_EtOH_5
Secondary FlyBase IDs

References ( 1 )
Personal communication to FlyBase	Graveley et al., 2011.4.14, The D. melanogaster transcriptome: modENCODE RNA-Seq data for differing treatment conditions The D. melanogaster transcriptome: modENCODE RNA-Seq data for differing treatment conditions [FBrf0213504]