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Dataset mE_mRNA_L3_EtOH_2.5
| General Information | |||
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| Name | mE_mRNA_L3_EtOH_2.5 | Species | D. melanogaster |
| Dataset type | RNA-Seq transcriptome coverage | FlyBase ID | FBlc0000256 |
| Source & Content | |||
| Consists of |
cDNA short sequencing reads from high-throughput sequencing.
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| Created by | |||
| Available from | |||
| Strain |
Oregon-R-modENCODE
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Stage & tissue | |
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Stage
Tissue/Position (including subcellular localization)
Reference
Comment:treatment: ethanol exposure (2.5%)
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| Cell Line |
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Recent Updates
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| Description |
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| FB2013_03 | |||
| FB2013_02 | |||
| All updates | Click here to see a list of all updates to this record from FB2010_08 and on. | ||
Description & Members
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| Description |
Short sequencing reads of cDNA prepared from polyadenylated RNA isolated from third instar larvae exposed to ethanol (2.5%
for 3 hours). Transcriptome represented as frequency of strand-specific reads along genome (see GBrowse presentation; data
in wiggle format); this collection is composed of uniquely aligned reads.
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| Parent collections |
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| Component collection(s) |
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| Number in collection |
96,879,760
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| Comment on number in collection |
Number of unique reads.
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| Members |
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Experimental protocol
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| Vector | |||
| Sample preparation |
Third instar larvae were cultured on food supplemented with 2.5% EtOH for 3 hours; 4.7% lethality was observed. Survivors
were flash frozen in liquid nitrogen and stored at -80 degrees C prior to RNA preparations. See report for modENCODE_mRNA-Seq_treatments for information on RNA isolation.
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| Collection preparation |
See report for modENCODE_mRNA-Seq_treatments.
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| Mode of assay | |||
| Assay platform |
See report for modENCODE_mRNA-Seq_treatments.
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| Data analysis |
See report for modENCODE_mRNA-Seq_treatments.
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Additional data
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| Associated files | |||
| Additional sites | |||
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Synonyms & Secondary IDs
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| Reported As | |||
| Symbol Synonym |
2.5% EtOH Third Instar larvae
EtOH_2.5_L3
mE_mRNA_L3_EtOH_2.5
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| Secondary FlyBase IDs | |||
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References
( 1 )
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| Personal communication to FlyBase |
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