General Information
Name
mE1_CBP_Enhancers
Species
D. melanogaster
Result type
FlyBase ID
FBlc0000415
Project
Data Provider
Title
Putative embryonic-specific enhancers identified by ChIP-chip and ChIP-Seq of nej (CBP) in embryos, larvae, pupae and adults.
Status
Current
Accessions
    Biosample Source
    Overview
    Tissue isolated
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    Sample preparation
    Biosamples analyzed by this result (24)
    Biosample
    Type
    Title
    D. melanogaster, iso-1 strain, embryo (0-4 hr AEL), source for ChIP.
    D. melanogaster, iso-1 strain, embryo (20-24 hr AEL), source for ChIP.
    D. melanogaster, iso-1 strain, embryo (0-4 hr AEL), source for ChIP.
    D. melanogaster, iso-1 strain, adult female, source for ChIP.
    D. melanogaster, iso-1 strain, adult male, source for ChIP.
    D. melanogaster, iso-1 strain, embryo (0-4 hr AEL), source for ChIP.
    D. melanogaster, iso-1 strain, adult female, source for ChIP.
    D. melanogaster, iso-1 strain, adult male, source for ChIP.
    D. melanogaster, iso-1 strain, embryo (0-4 hr AEL), source for ChIP.
    D. melanogaster, iso-1 strain, embryo (12-16 hr AEL), source for ChIP.
    Showing 10 / 24 records. Use Export to HitList above to see all
    Data Analyzed
    Key genes
    (bait_protein)
    Protocol
    Mode of Assay
    Raw Data Analyzed (24)
    Assay / Reagent collection
    Type
    Title
    ChIP-chip of nej in D. melanogaster, iso-1 strain, embryo (0-4 hr AEL).
    ChIP-chip of nej in D. melanogaster, iso-1 strain, embryo (4-8 hr AEL).
    ChIP-chip of nej in D. melanogaster, iso-1 strain, embryo (16-20 hr AEL).
    ChIP-chip of nej in D. melanogaster, iso-1 strain, embryo (0-4 hr AEL).
    ChIP-chip of nej in D. melanogaster, iso-1 strain, embryo (12-16 hr AEL).
    ChIP-Seq of nej in D. melanogaster, iso-1 strain, embryo (0-4 hr AEL).
    ChIP-Seq of nej in D. melanogaster, iso-1 strain, embryo (12-16 hr AEL).
    ChIP-Seq of nej in D. melanogaster, iso-1 strain, embryo (16-20 hr AEL).
    ChIP-Seq of nej in D. melanogaster, iso-1 strain, embryo (20-24 hr AEL).
    ChIP-Seq of nej in D. melanogaster, iso-1 strain, embryo (0-4 hr AEL).
    Showing 10 / 24 records. Use Export to HitList above to see all
    Processed Data Analyzed (0)
    Result
    Type
    Title
    Analysis
    Methods
    Reference Genome
    Reference Annotation
    Data analysis
    Peak calling for ChIP-chip experiments was performed using MAT (Johnson et al. 2006. Proc. Natl. Acad. Sci. U.S.A. 103(33): 12457-12462). Peak calls from ChiP-Seq data were performed using MACS (Zhang et al. 2008. Genome Biol. 9(9): R137) or Peakseq (Rozowsky et al. 2009. Nat. Biotechnol. 27(1): 66-75). Peaks from CBP ChIP-chip and ChIP-seq experiments were filtered to exclude peaks falling between -500bp and +500bp of annotated promoters. CBP binding sites occuring at only one of the 12 developmental stages analyzed were also removed. Peaks were merged and a final list of sites occuring only during embryogenesis was generated.
    Comments
    Associated Data
    Size
    Associated features
    81 enhancer(s)
    Files
    Additional Information
    Synonyms and Secondary IDs (6)
    Reported As
    Symbol Synonym
    CBP binding sites
    CBP embryo only enhancer predictions
    Embryo-specific CBP binding
    mE1_CBP_Enhancers
    Name Synonyms
    Putative embryonic-specific enhancers identified by ChIP-chip and ChIP-Seq of nej (CBP) in embryos, larvae, pupae and adults.
    Secondary FlyBase IDs
      References (4)