General Information
Name
TRiP-5
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000416
Project
Created by
Title
A set of transgenic RNAi constructs for expression of shRNA under UAS control, TRiP second generation (pVALIUM21).
Accessions
    Overview
    Description
    The TRiP-5 construct collection represents a second generation of TRiP lines. Each construct is designed to target the knockdown of a single gene during oogenesis using short hairpin RNA (shRNA).
    NOTE: Dataset members correspond to the amplicon sequence features; these can be used to retrieve associated constructs and stocks.
    Biosample Source
    Overview
    Strain
    Stage
    Sex
    Tissue isolated
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    Sample preparation
    Reagent Details
    Methods
    Molecular Construct used
    Transgenic Construct used
    Protocol
    Transgenic animals were designed to carry a short hairpin (shRNA) microRNA under UAS-GAL4 control. These shRNA are inserted into pVALIUM21, which is a variant of pVALIUM22 (TRiP-4) that lacks the ftz intron and gypsy sequences.
    Mode of Assay
    Data analysis
    Comments
    Constructs of the TRiP-5 collection, based on pVALIUM21, are designed to trigger effective RNAi knock-down in the female germline. The P-transposase core promoter in pVALIUM21-based constructs directs little expression in somatic cells.
    Associated Data
    Size
    Associated features
    94 RNAi_reagent(s)
    Files
    Additional Information
    [TRiP Approach - 2nd generation](http://www.flyrnai.org/TRiP-TTR-GEN2.html)
    Synonyms and Secondary IDs (2)
    Reported As
    Symbol Synonym
    Name Synonyms
    A set of transgenic RNAi constructs for expression of shRNA under UAS control, TRiP second generation (pVALIUM21).
    Secondary FlyBase IDs
      References (4)