Dataset NIH_Dsim_2_F

General Information
Name	NIH_Dsim_2_F	Species	D. simulans
Dataset type	RNA-Seq transcriptome coverage	FlyBase ID	FBlc0000430
Source & Content
Consists of	cDNA short sequencing reads from high-throughput sequencing. (Malone et al., 2011.8.9)
Created by
Available from
Strain
Stage & tissue
Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & female -- day 7 of adulthood & female organism (Malone et al., 2011.8.9)
Cell Line
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Members
Description	Short sequencing reads of cDNA prepared from polyadenylated RNA isolated from Drosophila simulans adult females. Transcriptome represented as frequency of reads along genome (see GBrowse presentation; data in wiggle format). (Malone et al., 2011.8.9)
Parent collections	NIH_mRNA_Dsim_adults
Component collection(s)
Number in collection	50,066,324 (Malone et al., 2011.8.9)
Comment on number in collection	Uniquely aligned reads; as reported. (Malone et al., 2011.8.9)
Members
Experimental protocol
Vector
Sample preparation	See report for NIH_mRNA_Dsim_adults. (Malone et al., 2011.8.9)
Collection preparation	See report for NIH_mRNA_Dsim_adults. (Malone et al., 2011.8.9)
Mode of assay	Read length (bases):75 (Malone et al., 2011.8.9) See report for NIH_mRNA_Dsim_adults. (Malone et al., 2011.8.9)
Assay platform	See report for NIH_mRNA_Dsim_adults. (Malone et al., 2011.8.9)
Data analysis	Used >3 offset reads. See report for NIH_mRNA_Dsim_adults. (Malone et al., 2011.8.9)
Additional data
	More information is available under: GEO: GSE28078
Associated files
Additional sites
Comments
	Strain used: 14021-0251.011 obtained from the Drosophila Species Stock Center (DSSC); previous stock designation (BGS) is w[501] X-chromosome. Strain used differs from the w[501] sequenced strain. (Malone et al., 2011.8.9)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	Dsim_251.011_F (Malone et al., 2011.8.9) NIH_Dsim_2_F
Secondary FlyBase IDs

References ( 1 )
Personal communication to FlyBase	Malone et al., 2011.8.9, mRNA-Seq of adult flies from the genus Drosophila mRNA-Seq of adult flies from the genus Drosophila [FBrf0214763]