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General Information
Name
Dfs_BSC_set1
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000496
Project
Created by
Vector
    Title
    A set of autosomal deficiencies created by deleting the region between two P-element insertions, filling in coverage gaps in other deficiency sets.
    Accessions
      Overview
      Description
      A set of ~50 targeted deficiencies created by exploiting hybrid element insertion (HEI) and resolution; designed to fill gaps in deletion coverage.
      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Transgenic Construct used
      Protocol
      Deletion-generation strategy based on the observation that when two P-element insertions are present at different sites on homologous chromosomes, upon exposure to P-transposase deletions that remove the region between the two insertions can be recovered. Resulting deletions are usually flanked by one of the starting P constructs, providing a molecular tag for subsequent breakpoint characterization; mechanism is thought to involve hybrid element insertion (HEI). Successfully used combinations of P{PZ}, P{lacW}, P{SUPor-P}, P{EP} and P{GT1} insertions. Recovered deletions whether the two P-element insertions were in the same or opposite orientations.
      Mode of Assay
      Data analysis
      The resulting deletion breakpoints do not correspond exactly with the locations of the original insertions, but are usually very close. This appears to be due to a strong inherent bias of hybrid elements to insert in or near one of the original P insertions and was confirmed by the observation that there were only 2 deletions recovered in the HEI screens for which the breakpoints differed from the sites of the original P insertions at the level of polytene cytology.
      Comments
      Associated Data
      Size
      Associated features
      53 Aberration(s)
      Additional Information
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      BSC Dfs
      Dfs_BSC_set1
      HEI deficiencies
      Name Synonyms
      A set of autosomal deficiencies created by deleting the region between two P-element insertions, filling in coverage gaps in other deficiency sets.
      Secondary FlyBase IDs
        References (3)