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General Information
Name
Dfs_BSC_set2
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000501
Project
Created by
Vector
    Title
    A set of deficiencies created by FLP-mediated recombination between FRT-carrying transgenic insertions, filling in coverage gaps in other deficiency sets.
    Accessions
      Overview
      Description
      A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Transgenic Construct used
      Protocol
      Deletion-generation strategy made use of extensive collections of isogenic FRT-carrying transgenic insertion lines (FBrf0175002); used insertions of P{XP}, PBac{RB} and PBac{WH}. Isogenized chromosomes were not used for all crosses involving the Y and 4th chromosomes. Recombination between FRT elements in two insertions in the appropriate relative orientation results in deletion of the genomic region between the insertions and production of a hybrid element that contains one FRT element at the recombination point (FBrf0091061). Animals were generated that carried a heat-shock-inducible FLP recombinase gene and 2 FRT-carrying transgenic insertions in trans; these were subjected to a heat-shock regimen during larval stages. After eclosion, females were collected and crossed to appropriate males for detection of miniwhite[-] or miniwhite[+] deficiency chromosomes; isolate lines were established using isogenized balancer stocks whenever possible. Four approaches were used to verify that the expected deletion was recovered from a screen: complementation tests, polytene chromosome cytology, hybrid PCR and PCR with primers flanking transposable element insertions. For the hybrid PCR method, IPCR primers within each FRT-bearing progenitor insertion can amplify a fragment only if they are juxtaposed by FLP-mediated recombination (are within the hybrid residual element); 93% of the deletions lacking miniwhite markers were tested by this method and all gave the expected result.
      Mode of Assay
      Data analysis
      A positive hybrid PCR test was used as the sole criterion for verifying only six deletions; all other deletions were also confirmed on the basis of deleted genes or sequences.
      Comments
      Associated Data
      Size
      Associated features
      793 Aberration(s)
      Additional Information
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      BSC Dfs
      Dfs_BSC_set2
      Name Synonyms
      A set of deficiencies created by FLP-mediated recombination between FRT-carrying transgenic insertions, filling in coverage gaps in other deficiency sets.
      Secondary FlyBase IDs
        References (3)