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General Information
Name
TI_set_P{lacW}.BDGP
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000509
Vector
    Title
    A set of lethal or sterile mutations associated with insertion of the P-element construct P{lacW}.
    Accessions
      Overview
      Description

      A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

      Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

      Available from
      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Transgenic Construct used
      Protocol

      A genetic screen allowing selection of autosomal insertions was used. Parental males carried a P{lacW} insertion on the X chromosome and the transposase-producing P{Δ2-3}99B construct on a marked third chromosome; they were crossed to w[-] females. F1 males that exhibited a mini-white phenotype were presumed to carry a new autosomal insertion; these were mated individually to determine the chromosomal location of the transposed insertion. Unless they differed significantly in eye color, multiple F1 males from the same parental vial were considered to share a common insertion (via a pre-mieotic cluster). Each line was tested for lethality and stable stocks established.

      A genetic screen for selection of second chromosome insertions was used. Parental males carried a P{lacW} insertion on the X chromosome and the transposase-producing P{Δ2-3}99B construct on a marked third chromosome; they were crossed en masse to w[-] females. F1 males that exhibited a mini-white phenotype were presumed to carry a new autosomal insertion; these were mated individually to determine the chromosomal location of the transposed insertion. Stable stocks were established for lethal and semi-lethal insertions on II. Pre-meiotic clusters were identified by complementation analyses between insertions arising in the same original cross.

      A genetic screen allowing selection of autosomal insertions was used, as described in FBrf0049800.

      An aggregate collection of lines produced in multiple different laboratories (see below). Additional lines contributed by M. Scott and M. Fuller (unpublished); no further information provided. From the screen described inFBrf0064397, only third chromosome insertions were used for this dataset.

      Markers in stocks (as originally submitted to the BDSC):y1 w* in the Jan ‘j’ lines; y1 w1118 in the Laughon ‘L’ lines; y1 w1118 in the other Jan ‘j’ lines; y1 w67c23 in the Kiss ‘k’ lines.

      Mode of Assay
      Data analysis

      General location of each insertion was determined by in situ hybridization to polytene chromosomes. Insertions that mapped to the same cytological location were tested for complementation. Using an array of characterized autosomal deficiencies, in many cases it was possible to test for complementation with an appropriate deficiency; failure to complement resulted in the line being characterized as "verified." For each line flanking genomic sequence was isolated by plasmid rescue (primarily) or by inverse PCR. Original collection consisted of 493 validated lines.

      378 lines were selected for inclusion in the BDGP Gene Disruption Project 2004 collection.

      Comments
      Associated Data
      Size
      Associated features
      1,843 Insertion(s)
      Files
      Additional Information
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      GDP_insertions_P{lacW}.BDGP
      TI_set_P{lacW}.BDGP
      Name Synonyms
      A set of lethal or sterile mutations associated with insertion of the P-element construct P{lacW}.
      Secondary FlyBase IDs
        References (9)