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General Information
Name
TI_set_P{EP}.EP
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000510
Vector
    Title
    A set of P-element construct P{EP} insertions.
    Accessions
      Overview
      Description

      A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w+mC mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.

      Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.

      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Transgenic Construct used
      Protocol

      Initial insertions were recovered after injection of a plasmid containing P{EP} into embryos; additional sites were recovered after exposure to the transposase-producing P{Δ2-3}99B construct on the third chromosome. Insertions were mapped to a chromosome and balanced; lines for which a single P-element was detected by Southern blot and by in situ hybridization to polytene chromosomes were characterized further.

      Mobilization of the P-element occurred in animals (both males and females were used) carrying P{EP} on a second chromosome CyO balancer chromosome and P{Δ2-3}99B, a stable source of P transposase, on a marked third chromosome. 2300 independent new inserts were mapped to chromosomes X, 2 or 3 and balanced for further analysis.

      Markers in stocks (as originally submitted to the BDSC):w1118.

      Mode of Assay
      Data analysis

      For 2266 lines generated in the Rorth laboratory, flanking genomic sequences were obtained by sequencing of inverse PCR products, and were mapped by alignment to the genomic sequence. 374 lines were selected for inclusion in the BDGP Gene Disruption Project 2004 collection.

      Comments

      The Scer\GAL4 driver in the P{EP} construct cannot be activated in the female germ line.

      Associated Data
      Size
      Associated features
      2,403 Insertion(s)
      Files
      Additional Information
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      GDP_insertions_P{EP}.EP
      TI_set_P{EP}.EP
      TI_set_P{EP}.Rorth
      Name Synonyms
      A set of P-element construct P{EP} insertions.
      Secondary FlyBase IDs
        References (7)